Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri

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Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri. / Chettri, Jiwan Kumar; Raida, Martin Kristian; Kania, Per Walter; Buchmann, Kurt.

I: Developmental & Comparative Immunology, Bind 36, Nr. 2, 2012, s. 463-474.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Chettri, JK, Raida, MK, Kania, PW & Buchmann, K 2012, 'Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri', Developmental & Comparative Immunology, bind 36, nr. 2, s. 463-474. https://doi.org/10.1016/j.dci.2011.08.014

APA

Chettri, J. K., Raida, M. K., Kania, P. W., & Buchmann, K. (2012). Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri. Developmental & Comparative Immunology, 36(2), 463-474. https://doi.org/10.1016/j.dci.2011.08.014

Vancouver

Chettri JK, Raida MK, Kania PW, Buchmann K. Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri. Developmental & Comparative Immunology. 2012;36(2):463-474. https://doi.org/10.1016/j.dci.2011.08.014

Author

Chettri, Jiwan Kumar ; Raida, Martin Kristian ; Kania, Per Walter ; Buchmann, Kurt. / Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri. I: Developmental & Comparative Immunology. 2012 ; Bind 36, Nr. 2. s. 463-474.

Bibtex

@article{422baa37d2634314815ce7001aec8124,
title = "Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri",
abstract = "Innate immune factors play a crucial role in survival of young fish especially during early stages of life whenadaptive immunity is not fully developed. In the present study, we investigated the immune response ofrainbow trout (Oncorhynchus mykiss) larvae and fry at an early stage of development. We exposed 17and 87 days post hatch larvae and fry (152 and 1118 degree days post hatch; avg. wt. 70 and 770 mg,respectively) to the bacterial pathogen, Yersinia ruckeri for 4 h by bath challenge. Samples were taken at4, 24, 72 and 96 h post exposure for qPCR and immunohistochemical analyses to elucidate the immuneresponse mounted by these young fish. Larvae showed no mortality although infected larvae at 48 h postexposure showed hyperaemia in the mouth region and inflammation on the dorsal side of the body. Geneexpression studies showed an up-regulation of iNOS and IL-22 in infected larvae 24 h post exposure butmost of the investigated genes did not show any difference between infected and uninfected larvae. Immunohistochemicalstudies demonstrated a high expression of IgT molecules in gills and CD8 positive cells inthymus of both infected and uninfected larvae. Infection of rainbow trout fry with Y. ruckeri, in contrast,induced a cumulative mortality of 74%. A high expression of cytokines (IL-1b, TNF-a, IL-22, IL-8 and IL-10), acute phase proteins (SAA, hepcidin, transferrin and precerebellin), complement factors (C3, C5 andfactor B), antimicrobial peptide (cathelicidin-2) and iNOS was found in infected fry when compared tothe uninfected control. IgT molecules and mannose binding lectins in gills of both infected and uninfectedfry were detected by immunohistochemistry. The study indicated that early life stages (yolk-sac larvae),merely up-regulate a few genes and suggests a limited capacity of larvae to mount an immune responseby gene regulation at the transcriptional level. Based on the observed clearance of bacteria and lack of mortalityit could be speculated that larvae may be covered by protective shield of different immune factorsproviding protection against broad range of pathogens. However, the increased susceptibility of older frysuggests that Y. ruckeri may utilize some of the immune elements to enter the naive fish. The up-regulationof iNOS and IL-22 in the infected larvae implicates an important role of these molecules in immuneresponse at early developmental stages. A dense covering of surfaces of gill filaments by IgT antibody inthe young fish suggest a role of this antibody as innate immune factor at early developmental stages.",
keywords = "Former LIFE faculty, Early life stages, Larvae, Innate immunity, I&T, CD8, Cytokines, Acute phase proteins, IL-22, iNOS",
author = "Chettri, {Jiwan Kumar} and Raida, {Martin Kristian} and Kania, {Per Walter} and Kurt Buchmann",
year = "2012",
doi = "10.1016/j.dci.2011.08.014",
language = "English",
volume = "36",
pages = "463--474",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Pergamon",
number = "2",

}

RIS

TY - JOUR

T1 - Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri

AU - Chettri, Jiwan Kumar

AU - Raida, Martin Kristian

AU - Kania, Per Walter

AU - Buchmann, Kurt

PY - 2012

Y1 - 2012

N2 - Innate immune factors play a crucial role in survival of young fish especially during early stages of life whenadaptive immunity is not fully developed. In the present study, we investigated the immune response ofrainbow trout (Oncorhynchus mykiss) larvae and fry at an early stage of development. We exposed 17and 87 days post hatch larvae and fry (152 and 1118 degree days post hatch; avg. wt. 70 and 770 mg,respectively) to the bacterial pathogen, Yersinia ruckeri for 4 h by bath challenge. Samples were taken at4, 24, 72 and 96 h post exposure for qPCR and immunohistochemical analyses to elucidate the immuneresponse mounted by these young fish. Larvae showed no mortality although infected larvae at 48 h postexposure showed hyperaemia in the mouth region and inflammation on the dorsal side of the body. Geneexpression studies showed an up-regulation of iNOS and IL-22 in infected larvae 24 h post exposure butmost of the investigated genes did not show any difference between infected and uninfected larvae. Immunohistochemicalstudies demonstrated a high expression of IgT molecules in gills and CD8 positive cells inthymus of both infected and uninfected larvae. Infection of rainbow trout fry with Y. ruckeri, in contrast,induced a cumulative mortality of 74%. A high expression of cytokines (IL-1b, TNF-a, IL-22, IL-8 and IL-10), acute phase proteins (SAA, hepcidin, transferrin and precerebellin), complement factors (C3, C5 andfactor B), antimicrobial peptide (cathelicidin-2) and iNOS was found in infected fry when compared tothe uninfected control. IgT molecules and mannose binding lectins in gills of both infected and uninfectedfry were detected by immunohistochemistry. The study indicated that early life stages (yolk-sac larvae),merely up-regulate a few genes and suggests a limited capacity of larvae to mount an immune responseby gene regulation at the transcriptional level. Based on the observed clearance of bacteria and lack of mortalityit could be speculated that larvae may be covered by protective shield of different immune factorsproviding protection against broad range of pathogens. However, the increased susceptibility of older frysuggests that Y. ruckeri may utilize some of the immune elements to enter the naive fish. The up-regulationof iNOS and IL-22 in the infected larvae implicates an important role of these molecules in immuneresponse at early developmental stages. A dense covering of surfaces of gill filaments by IgT antibody inthe young fish suggest a role of this antibody as innate immune factor at early developmental stages.

AB - Innate immune factors play a crucial role in survival of young fish especially during early stages of life whenadaptive immunity is not fully developed. In the present study, we investigated the immune response ofrainbow trout (Oncorhynchus mykiss) larvae and fry at an early stage of development. We exposed 17and 87 days post hatch larvae and fry (152 and 1118 degree days post hatch; avg. wt. 70 and 770 mg,respectively) to the bacterial pathogen, Yersinia ruckeri for 4 h by bath challenge. Samples were taken at4, 24, 72 and 96 h post exposure for qPCR and immunohistochemical analyses to elucidate the immuneresponse mounted by these young fish. Larvae showed no mortality although infected larvae at 48 h postexposure showed hyperaemia in the mouth region and inflammation on the dorsal side of the body. Geneexpression studies showed an up-regulation of iNOS and IL-22 in infected larvae 24 h post exposure butmost of the investigated genes did not show any difference between infected and uninfected larvae. Immunohistochemicalstudies demonstrated a high expression of IgT molecules in gills and CD8 positive cells inthymus of both infected and uninfected larvae. Infection of rainbow trout fry with Y. ruckeri, in contrast,induced a cumulative mortality of 74%. A high expression of cytokines (IL-1b, TNF-a, IL-22, IL-8 and IL-10), acute phase proteins (SAA, hepcidin, transferrin and precerebellin), complement factors (C3, C5 andfactor B), antimicrobial peptide (cathelicidin-2) and iNOS was found in infected fry when compared tothe uninfected control. IgT molecules and mannose binding lectins in gills of both infected and uninfectedfry were detected by immunohistochemistry. The study indicated that early life stages (yolk-sac larvae),merely up-regulate a few genes and suggests a limited capacity of larvae to mount an immune responseby gene regulation at the transcriptional level. Based on the observed clearance of bacteria and lack of mortalityit could be speculated that larvae may be covered by protective shield of different immune factorsproviding protection against broad range of pathogens. However, the increased susceptibility of older frysuggests that Y. ruckeri may utilize some of the immune elements to enter the naive fish. The up-regulationof iNOS and IL-22 in the infected larvae implicates an important role of these molecules in immuneresponse at early developmental stages. A dense covering of surfaces of gill filaments by IgT antibody inthe young fish suggest a role of this antibody as innate immune factor at early developmental stages.

KW - Former LIFE faculty

KW - Early life stages

KW - Larvae

KW - Innate immunity

KW - I&T

KW - CD8

KW - Cytokines

KW - Acute phase proteins

KW - IL-22

KW - iNOS

U2 - 10.1016/j.dci.2011.08.014

DO - 10.1016/j.dci.2011.08.014

M3 - Journal article

VL - 36

SP - 463

EP - 474

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 2

ER -

ID: 35243505